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<t>Mdivi-1</t> <t>treatment</t> impairs creatine kinase B (Ckb)-mediated osteogenic differentiation of bone marrow mesenchymal stem cells (BMSCs). (A) CCK-8 assay evaluated the cytotoxicity of Mdivi-1. (B-D) mRNA expression levels of osteogenesis-related genes ( Alp, Runx2 , and Opn ) after Mdivi-1 treatment. (E, F) Protein expression levels of osteogenic markers (Alp, Runx2, and Opn) via Western blot (E) and quantitative gray value analysis (F) at 7 days of induction. (G) ALP staining after 7 days of induction. (H) ARS staining after 14 days of induction. (I) Quantitative analysis of ALP activity. (J) Quantitative analysis of calcium deposition. All data represent mean ± SD, n = 3. Statistical significance: * P < .05, ** P < .01, *** P < .001, **** P < .0001.
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<t>Mdivi-1</t> <t>treatment</t> impairs creatine kinase B (Ckb)-mediated osteogenic differentiation of bone marrow mesenchymal stem cells (BMSCs). (A) CCK-8 assay evaluated the cytotoxicity of Mdivi-1. (B-D) mRNA expression levels of osteogenesis-related genes ( Alp, Runx2 , and Opn ) after Mdivi-1 treatment. (E, F) Protein expression levels of osteogenic markers (Alp, Runx2, and Opn) via Western blot (E) and quantitative gray value analysis (F) at 7 days of induction. (G) ALP staining after 7 days of induction. (H) ARS staining after 14 days of induction. (I) Quantitative analysis of ALP activity. (J) Quantitative analysis of calcium deposition. All data represent mean ± SD, n = 3. Statistical significance: * P < .05, ** P < .01, *** P < .001, **** P < .0001.
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<t>Mdivi-1</t> <t>treatment</t> impairs creatine kinase B (Ckb)-mediated osteogenic differentiation of bone marrow mesenchymal stem cells (BMSCs). (A) CCK-8 assay evaluated the cytotoxicity of Mdivi-1. (B-D) mRNA expression levels of osteogenesis-related genes ( Alp, Runx2 , and Opn ) after Mdivi-1 treatment. (E, F) Protein expression levels of osteogenic markers (Alp, Runx2, and Opn) via Western blot (E) and quantitative gray value analysis (F) at 7 days of induction. (G) ALP staining after 7 days of induction. (H) ARS staining after 14 days of induction. (I) Quantitative analysis of ALP activity. (J) Quantitative analysis of calcium deposition. All data represent mean ± SD, n = 3. Statistical significance: * P < .05, ** P < .01, *** P < .001, **** P < .0001.
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Mdivi-1 treatment impairs creatine kinase B (Ckb)-mediated osteogenic differentiation of bone marrow mesenchymal stem cells (BMSCs). (A) CCK-8 assay evaluated the cytotoxicity of Mdivi-1. (B-D) mRNA expression levels of osteogenesis-related genes ( Alp, Runx2 , and Opn ) after Mdivi-1 treatment. (E, F) Protein expression levels of osteogenic markers (Alp, Runx2, and Opn) via Western blot (E) and quantitative gray value analysis (F) at 7 days of induction. (G) ALP staining after 7 days of induction. (H) ARS staining after 14 days of induction. (I) Quantitative analysis of ALP activity. (J) Quantitative analysis of calcium deposition. All data represent mean ± SD, n = 3. Statistical significance: * P < .05, ** P < .01, *** P < .001, **** P < .0001.

Journal: Stem Cells Translational Medicine

Article Title: Ckb-Cryab-Drp1 axis enhances BMSC osteogenic differentiation and mouse alveolar bone healing through mitochondrial fission modulation

doi: 10.1093/stcltm/szag056

Figure Lengend Snippet: Mdivi-1 treatment impairs creatine kinase B (Ckb)-mediated osteogenic differentiation of bone marrow mesenchymal stem cells (BMSCs). (A) CCK-8 assay evaluated the cytotoxicity of Mdivi-1. (B-D) mRNA expression levels of osteogenesis-related genes ( Alp, Runx2 , and Opn ) after Mdivi-1 treatment. (E, F) Protein expression levels of osteogenic markers (Alp, Runx2, and Opn) via Western blot (E) and quantitative gray value analysis (F) at 7 days of induction. (G) ALP staining after 7 days of induction. (H) ARS staining after 14 days of induction. (I) Quantitative analysis of ALP activity. (J) Quantitative analysis of calcium deposition. All data represent mean ± SD, n = 3. Statistical significance: * P < .05, ** P < .01, *** P < .001, **** P < .0001.

Article Snippet: Mdivi-1 (MedChemExpress, USA) was dissolved in dimethyl sulfoxide (DMSO) as a stock solution and stored at −20 °C.

Techniques: CCK-8 Assay, Expressing, Western Blot, Staining, Activity Assay